CD68 Antikörper

Produktnummer ABIN111830

Der Maus Monoklonal Anti-CD68-Antikörper wurde für FACS, WB, IF, IHC (p), IP, IHC (fro) und RIA validiert. Er ist geeignet, CD68 in Proben von Ratte zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für CD68 Antikörper (ABIN111830)

Target: CD68 (CD68 Molecule (CD68))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD68 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Klon: ED1

Produktdetails anti-CD68 Antikörper

Spezifität: CD68 Antibody (Cat#BM4000) recognises a single chain glycoprotein of 110 kDa that is expressed predominantly on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes.

Kreuzreaktivität (Details): Species reactivity (tested):Rat.

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Rat spleen cells. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: RIA. Western blotting. Immunoprecipitation. Flow Cytometry: Use 10 μL of 1/50-1/100 diluted CD68 antibody to label 10^6 cells in 100 μL(Results may be enhanced using membrane permeabilisation). Immunohistology on Frozen Sections. Immunohistology on Paraffin Sections (1/100): Requires protein digestion pretreatment ofParaffin sections with Pronase or Trypsin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-CD68 Antikörper (ABIN111830)

Meyer, Cunea, Sonntag-Bensch, Welker, Licha, Holz, Schmitz-Valckenberg: "In vivo imaging of a new indocyanine green micelle formulation in an animal model of laser-induced choroidal neovascularization." in: Investigative ophthalmology & visual science, Vol. 55, Issue 10, pp. 6204-12, (2014) (PubMed).

Schneider, Meusel, Renker, Bauer, Holzinger, Roeder, Wanner, Gekle, Sauvant: "Low-dose indomethacin after ischemic acute kidney injury prevents downregulation of Oat1/3 and improves renal outcome." in: American journal of physiology. Renal physiology, Vol. 297, Issue 6, pp. F1614-21, (2009) (PubMed).

Details zu CD68

Target: CD68 (CD68 Molecule (CD68))

Andere Bezeichnung: CD68

Hintergrund: The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.Synonyms: Gp110, Macrophage marker, Macrosialin

Gen-ID: 287435

NCBI Accession: NP_001026808

UniProt: Q4FZY1